Welcome to m6AConquer !

N6-methyladenosine (m6A) is the most prevalent RNA modification in mammalian cells and the most extensively studied epitranscriptomic mark. The m6AConquer project (Consistent Quantification of External m6A RNA Modification Data) summarizes quantitative m6A data from 10 detection techniques using 2,788,705 consistent base-resolution features.

Available m6A detection techniques fall into 4 categories: antibody-assisted, chemical-assisted, enzyme-assisted, and direct-RNA sequencing. m6AConquer leverages this categorization to develop a model-based integration framework that enables the faithful detection of 146,250 high-confidence m6A sites reproducible across orthogonal techniques. Additionally, m6AConquer standardizes site calling and m6A probability normalization across platforms using a statistical method that accounts for over-dispersion in m6A level. The current version of m6AConquer archived 1,928 sequencing samples, 72 cell lines or tissues, and 11 effectively integrated orthogonal technique pairs.