Welcome to m6AConquer !

N6-methyladenosine (m6A) is the most prevalent RNA modification in mammalian cells and the most extensively studied epitranscriptomic mark. The m6AConquer project (Consistent Quantification of External m6A RNA Modification Data) summarizes quantitative m6A data from 10 detection techniques using 2,788,705 (human) and 2,761,121 (mouse) consistent base-resolution features.

Available m6A detection techniques fall into 4 categories: antibody-assisted, chemical-assisted, enzyme-assisted, and direct-RNA sequencing. m6AConquer leverages this categorization to integrate and identify 139,700 high-confidence m6A sites reproducible across orthogonal techniques in human and mouse. Additionally, m6AConquer consistenly quantifies m6A profiling data and unifies site calling procedures, providing a standardised data-sharing framework. 4 types of multi-omics data, including m6A methylomes, expression levels, alternative splicing events, and variant calling results, are embedded into this framework. Using m6A methylome and genetic variant data, m6AConquer provides 4,506 m6A QTL locations. The current version of m6AConquer archived 1,928 FASTQ and FAST5 data, 1,394 sequencing samples, 72 cell lines or tissues, and 13 effectively integrated orthogonal technique pairs.